Author  
Place of duty  
Title   말초혈액 조혈모세포의 4℃ 냉장보존이 조혈능에 미치는 영향 ( Hematopoietic Recovery of Peripheral Blood Stem Cells Stored at 4℃ )
Publicationinfo   2000 Jan; 032(03): 647-655.
Key_word   Peripheral blood stem cell , Cryopreservation , 4℃
Full-Text  
Abstract   Purpose: Cryopreservation has been the standard method of storing hematopoietic cells for the past 20 years, but this procedure is laborious and expensive. So, we evaluated the hematopoietic recovery of stored PBSCs at 4C for a variable storage period Materials and Methods: Eight leukapheresis products were kept unprocessed at 4℃ for 96 hours. To evaluate the effect of storage period on the hematopoietic recovery of PBSCs, assays for viability of mononuclear cells (MNCs), CFU-GM colony counts and CD34+cell counts were performed every 24 hours after PBSC collection. We tried to compare hematopoetic recovery of stored PBSCs at 4℃ with that of cryopreserved PBSCs by using repeated measures ANOVA. Results: Viability of MNCs, CFU-GM' colony counts and CD34+cell counts were monitored at 24 hour, 48 hour, 72 hour and 96 hour after PBSC collection. Data are expressed as percentage of baseline value and shown as means,d.; MNCs viability (96±2%, 94±2%, 92±2%, 88± 3%), CFU-GM colony counts (87±10%, 79±11%, 65±13%, 56±15%), and CD34+ cell counts (93±13%, 93±12%, 88±14%, 85±19%). After storing PBSCs at 4C for 96 hours, viability of MNCs and CFU-GM colony counts were significantly reduced (p<0.05) except CD34+ cell concentration (p > 0.05). Procedures of controlled-rate freezing and thawing resulted in a notable loss of viability (77±9%) and CFU-GM colony count (71±29%), CFU-GM colony counts of 72 hour-stored PBSCs at 4C was similar to those of cryopreserved PBSCs. Conclusion: If G-CSF mobilized PBSCs are stored at 4C in less than 72 hours after collection, those hematopoie1ic recovery would be comparable to that of cryopreserved stem cells which are achieved by the rate-control freezer.
저 자   조석구(Seok Goo Cho), 오은지(Eun Jee Oh),이준모(Jun Mo Lee),김훈교(Hoon Kyo Kim),이경식(Kyung Shick Lee),김춘추(Chun Choo Kim)